... understanding life in molecular detail

EM People

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Professor Neil Ranson, Director of Electron microscopy

As Director of Electron microscopy at the Astbury Biostructure Laboratory, I’m responsible for the Academic leadership of the Facility, and am here to help with advice about all aspects of cryo-electron microscopy, from imaging and structure determination, to funding and costs. My laboratory is interested in the structures of macromolecular complexes and how conformational change drives biological function. There are currently projects ranging from large icosahedral (and pleomorphic) viruses, through amyloid protein aggregates to small membrane proteins.

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Dr Peiyi Wang, Facility Manager

As Facility Manager I ensure the smooth running of the electron microscopy facility. I come from a material sciences background, and so my focus is on ensuring the cryo-electron microscopes here can generate the highest possible resolution data. I train users in cryo-electron microscopy, and assist with cryo-electron microscopy data collection by single particle, tomographic and diffractions methods. 

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Dr Rebecca Thompson, Cryo-electron Microscopy Support Scientist

My role is to pump prime research using the cryo-electron microscopy facilities at the Astbury BioStructure Laboratory. I can assist in project design, cryogenic sample preparation, the collection of preliminary data for grant applications, and data processing.  I am particularly interested in pushing the boundaries of what can be achieved using electron microscopy, including tacking challenging complexes by single particle cryo-electron microscopy, as well as exploring the use of cryo-electron tomography to do structural biology in situ.

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Martin Fuller, Electron Microscopy Technician

My role is to help run the EM facility here at Leeds, supporting all users of the equipment in the facility. I train users in ambient transmission electron microscopy, as well as SEM. I can also help train users to prepare their samples by negative staining, and prepare embedded and sectioned material for ambient transmission electron microscopy, as well as sample preparation for scanning electron microscopy (SEM), cryo-SEM and environmental SEM. 

Group leaders with a major interest in electron microscopy

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Dr Stephen Muench

My group are interested in using EM in combination with X-ray crystallography and biochemical analysis to resolve the structure and mechanism of protein complexes, in particular membrane proteins. More recently we have been using EM to characterise small molecule binding and developing it as a tool for rational drug design.  We work on a number of systems including the vacuolar ATPase, ion channels and membrane transporters. 

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Dr Juan Fontana

The research in my lab focuses on understanding the molecular mechanisms of the entry, replication and morphogenesis of enveloped viruses. Our approach is mainly based on electron microscopy techniques, like cellular electron-microscopy, cryo-electron microscopy/tomography and subtomogram averaging. These techniques are combined with other structural and biochemical approaches.