... understanding life in molecular detail

The Radford Research Group

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Group Leader - Prof. Sheena E. Radford, FMedSci FRS
Research Interests: Protein Folding and Misfolding.
BSc in Biochemistry, University of Birmingham; PhD Biochemistry, University of Cambridge; Royal Society University Research Fellow (Protein Folding) in the Oxford Centre for Molecular Sciences; University Lecturer in the School of Biochemistry & Molecular Biology, University of Leeds; Reader in Structural Molecular Biology, University of Leeds; Professor of Structural Molecular Biology, University of Leeds.

Contact details:
Astbury Centre of Structural Molecular Biology, Garstang Building, University of Leeds, Leeds, LS2 9JT
Tel: 0113 343 3170; FAX: 0113 343 3167;
email: s.e.radford@leeds.ac.uk

Current Group Members

Helen McAllister
Helen is Prof Radford's PA. She organises the group and keeps everything coordinated. She can be contacted on h.mcallister@leeds.ac.uk 0113 343 7036
Helen McAllister
Ciaran Doherty
Ciaran graduated from the University of Leeds in Biological Sciences in 2013. He is supervised jointly with Dr David Brockwell funded by a BBSRC CASE award with UCB. He is using force spectroscopy to measure protein-protein interactions. He will join the ERC team in October 2017 as a postdoc to continue his studies on early aggregation events in amyloid formation using dynamic force spectroscoopy.
Ciaran Doherty
Dr Amit Kumar
Amit joined the group in 2014 having completed a postdoc in protein structure/function in Germany. He is working on a collaborative project on the role of flow in enhancing protein aggregation. The project is joint between our lab and those of Dr David Brockwell and Prof Nik Kapur (Faculty of Engineering).
Dr Amit Kumar
Dr Martin Walko
Martin joined the group in 2017 funded by EPSRC. His project is collaborative with Prof Andy Wilson (Chemistry), Prof Alison Ashcroft (mass spec) and Dr Eric Hewitt (cell biology). Martin's synthetic chemistry skills are being used to synthesise Aβ40/42 containing amino acids modified by cross linkers. His project aims to determine the structure and toxicity of Aβ aggregation intermediates.
Dr Martin Walko
Dr Matthew Iadanza
Matt joined the lab in 2014 having obtained his PhD from the University of Washington. He is also a member of the ERC team and works jointly with Dr Neil Ranson developing the use of cryo-electron microscopy and tomography to investigate amyloid fibril structures. From April 2018 Matt will work also on cryo-EM studies of the BAM complex funded by an MRC programme grant in collaboration with Prof Neil Ranson.
Dr Matthew Iadanza
Dr Nicolas Guthertz
Nicolas joined the group in August 2017 having completed his PhD at ICR in London. His skills in protein crystallography and protein-ligand interactions are now being applied to biochemical and kinetic analysis of the early stages in the aggregation of β2-microglobulin and amylin. He is funded by the Wellcome Trust.
Dr Nicolas Guthertz
Anna Higgins
Anna joined our Wellcome Trust funded PhD programme in 2014 having completed her degree in Biochemistry at the University of Edinburgh. She is now working on the mechanism of the β-barrel assembly machinery (BAM) using fluorescence and force methods. She is jointly supervised with Dr David Brockwell.
Anna Higgins
Atenas Posada Borbon
Atenas joined the group in 2016 and is jointly supervised by Dr Eric Hewitt. She joined us funded by a Mexican Fellowship having completed a Masters in Neuroscience from the Universite Pierre et Marie Curie in Paris. Continuing her interests in amyloid pathology she is investigating how cells respond to the presence of Aβ aggregates.
Atenas Posada Borbon
Dr Antonio Calabrese
Anton is a postdoc in the group funded by BBSRC. He works on the analysis of membrane protein structure, folding and dynamics using mass spectrometry, focussing on the BAM complex and the interaction with the chaperones SurA and Skp. He first joined the lab in 2013 having completed his PhD in the School of Chemistry and Physics at the University of Adelaide, Australia.
Dr Antonio Calabrese
Dr Chinar Pathak
Chinar joined the group in March 2017. He completed his PhD and postdoc in Korea using NMR and X-ray crystallography. Funded by the Wellcome Trust, he is exploring the use of protein affimers as anti-amyloid agents.
Dr Chinar Pathak
Dr Matthew Jackson
Matt joined the lab in 2014 as a postdoctoral researcher funded by ERC and jointly supervised by Prof Radford and Dr Eric Hewitt. He is using cell biological methods to investigate how amyloid fibrils and their assembly intermediates interact with, and disrupt, cellular homeostasis.
Dr Matthew Jackson
Dr Robert Schiffrin
Bob graduated in Biochemistry at the University of Leeds in July 2012 and joined the group as a PhD student to study the mechanisms of outer membrane protein folding and the involvement of molecular chaperones in this process. Jointly supervised by Dr David Brockwell, he is now a postdoc funded by BBSRC and is studying the molecular mechanism of BAM using a suite of biophysical approaches.
Dr Robert Schiffrin
Emma Cawood
Emma is a Wellcome Trust PhD student who joined the group in 2016 with a BSc in Biomolecular Science from the University of St.Andrews. Co-supervised by Prof Andy Wilson in the School of Chemistry, she is using ligand screening to develop inhibitors of protein-protein interactions in aggregation of proteins such as β2-microglobulin.
Emma Cawood
Hugh Smith
Hugh joined our Wellcome Trust 4-year PhD programme in 2014 having completed his degree in Biochemistry at the University of Bristol. His project is to use NMR and other biophysical methods to unravel the mechanism of amyloid formation of β2-microglobulin; including in hibition by small molecule and other reagents. He is jointly supervised by Prof Alex Breeze.
Hugh Smith
Nasir Khan
Nasir joined the group in 2010 as our lab manager. He keeps the lab running smoothly and hence is a key member of our research team. He has excellent skills in maintaining and developing our excellent equipment base and also works as the facility manager for the CD facility.
Nasir Khan
Sam Bunce
Sam joined the group in October 2014 funded by the White Rose BBSRC DTP. He is supervised jointly with Prof Andy Wilson (Chemistry) and Prof Alison Ashcroft (mass spec). His project combines synthesis of photoactivatable crosslinkers and peptide synthesis to determine the nature of intermediates in Aβ peptide assembly.
Sam Bunce
Sarah Good
Sarah started as a PhD student in October 2016 having graduated with a degree in Biological Sciences from the University of Leeds. She is funded by the BBSRC White Rose DTP. Co-supervised by Dr Patricija vanOosten-Hawle she will bring expertise in using C. elegans as a model for amyloid disease and its inhibition using small molecules and other strategies.
Sarah Good
Jessica Ebo
Jess started as a PhD student in the lab in October 2016 after graduating with a BSc in Biochemistry from the University of Leeds. She is a BBSRC iCASE student with Medimmune. Co-supervised with Dr David Brockwell she is developing new assays for evolving proteins as better biologics with enhanced bioprocessing properties.
Jessica Ebo
Jim Horne
Jim is supervised jointly with Dr David Brockwell.He joined the lab in 2015 after graduating with a MSc in Biosciences from the University of Bath. He is funded by the BBSRC White Rose DTP. His project focuses on how molecular chaperones assist OMP-folding and delivery to the BAM complex.
Jim Horne
Julia Humes
Julia joined the lab in 2014 after her degree in Biochemistry at the University of Newcastle. She is funded by the NIH in collaboration with Dr Jim Bardwell at the University of Michigan. Her project aims to elucideate the mechanism by which molecular chaperones in the E. coli periplasm facilitate folding in the absence of ATP.
Julia Humes
Lucy Barber
Lucy is a BBSRC-DTP student jointly supervised by Dr Anastasia Zhuravleva. She is working on a project to discover new small molecule inhibitors of amyloid formation and to elucide their mechanism of action using NMR and other biophysical methods. Lucy graduated in Biochemistry from the University of Oxford in 2014.
Lucy Barber
Michael Davies
Michael is a PhD student funded by the BBSRC White Rose DTP who started in the lab in October 2016. Co-supervised by Dr Eric Hewitt, his project focusses on the cell biology of amyloidosis, particularly how cells respond to the presnce of oligomeric and fibrillar aggregates. He joined us having completed his BSc in Veterinary Science at the Royal Veterinary College.
Michael Davies
Owen Cornwell
Owen joined the group in Jan 2016 having studied Biochemistry for his BSc at the University of Essex. He joined the group having worked with Medimmune/University of Warwick on mass spectrometry of proteins. Owen is funded by a BBSRC ICP award in partnership with Medimmune. He will be using and developing MS methods combined with other biophysical methods to study the aggregation of antibodies and other proteins. He is jointly supervised by Alison Ashcroft.
Owen Cornwell

Associated with the Group

Dr David Brockwell
David is an Associate Professor in the Faculty of Biological Sciences and a member of the Astbury Centre. More information can be found at http://www.fbs.leeds.ac.uk/staff/Brockwell_DJ/
Dr David Brockwell

Past Group Members - where are they now?

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We would like to thank the following organisations for the funds to support our research:
BBSRC, MRC, EPSRC, The Royal Society, The Wellcome Trust, and The University of Leeds.