Astbury Centre for Structural Molecular Biology
Annual Report 2003

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INDEX TO THE 2003 REPORT

The full 2003 report can be downloaded as an 11.5MB pdf filehere

Individual reports can be downloaded as pdf files using the links below

Director's statement for 2003 - Download

Ashcroft - Mass spectrometry facility

Ashcroft - Investigating the structure of monomeric and fibrillar beta2-microglobulin by limited proteolysis and mass spectrometry

Baron - Analytical centrifuge facility

Berry - Directed evolution of sialic acid synthesising enzymes

Berry - Directed evolution of dihydrodi picolinate synthase (DHDPS)

Berry - Directed evolution of an aldolase to enhance solubility

Findlay - Structure/function of G-protein coupled receptors (GPCRs)

Harris - Functional studies on the HIV-1 Nef protein

Harris - Structural and functional studies on the Hepatitis C virus non-structural NS5A protein

Homans - Thermodynamics of binding of 2-methoxy-3-isopropylpyrazine and 2-methoxy-3-isobutylpyrazine to the major urinary protein

Homans - Dissecting the cholera toxin-ganglioside GM1 interaction by isothermal titration calorimetry

Homans - The NMR solution structure of the VMA-7 subunit of the vacuolar H+-ATPase from Saccharomyces cerevisiae

Homans - Large scale millisecond inter-subunit dynamics in the B subunit homopentamer of the toxin derived form E. coli O157

Henderson - Collecting bacterial membrane proteins

Henderson - Characterisation of ligand binding to membrane transport proteins from Escherichia coli by solid state NMR

Hiscox - Characterisation of the coronavirus RNA binding protein

Jackson - Molecular modelling of SH2 domain-peptide interactions

Jackson - Flexligdock: A flexible ligand docking tool

Jackson - Searchable database containing comparisons of ligand binding sites at the molecular level for the discovery of similarities in protein function

Jackson - Structural modelling of protein-DNA interactions

Jaeger - Structural studies of RNA-directed RNA polymerases from Hepatitis C virus and related viruses

Jaeger - Biochemical and structural studies of Human Rhinovirus 3D polymerase

Jaeger - Functional and structural analysis of motif F in viral RNA polymerase

Jaeger - The mechanism of initiation in Hepatitis C virus RNA polymerase

Jaeger - Structural and biophysical studies on HIV-1 Nef

Kalverda - The NMR facility

McDowall - Quaternary structure and catalytic activity of the escherichia coli ribonuclease E amino-terminal catalytic domain

McDowall - Expanding the use of zymography by the chemical linkage of small, defined substrates to the gel matrix

McPherson - Enhanced enzyme properties by protein engineering of galactose oxidase

McPherson - Mobile TPQ cofactor and metals in E. coli copper amine oxidase

Nelson - Synthesis of screening substrates for the directed evolution of sialic acid aldolase

Parker - A combinatorial selective labelling methods for the assignment of backbone NMR resonances

Parker - Water as a conformational editor in protein folding

Phillips - Crystal structures of Qbeta RNA stemloop operators complexed with bacteriophage MS2 coat protein mutants; towards an explanation of binding discrimination

Phillips - Structural determination of human ketohexokinase

Phillips-Jones - Cytolysin gene expression in Enterococcus faecalis is regulated in response to aerobiosis conditions

Phillips-Jones - Expression, purification and characterisation of full-length, histidine protein kinase RegB (PrrB) from Rhodobacter sphaeroides

Phillips-Jones - Solution structure and DNA binding of the effector domain from the global regulator PrrA (RegA) from Rhodobacter sphaeroides: Insights into DNA binding specificity

Radford - Amyloid formation of peptides and point mutations of beta2-microglbulin

Radford - Investigating amyloid self-assembly by atomic force microscopy

Radford - Characterising the factors that describe the mechanical resistance of proteins

Radford - Mapping the folding landscapes of the four-helix proteins Im7 and Im9

Ranson - Cryo-EM of macromolecular complexes

Rowlands - Targeting the Hepatitis C virus ion channel p7 for anti-viral therapy

Rowlands - Discrimination among rhinovirus serotypes for a variant ICAM-1 receptor molecule

Smith - Atomic force microscopy of microdomains in lipid bilayers

Smith - The dynamic response of biomolecules

Smith - Fast folding of B domain of Protein A by laser induced temperature jump

Smith - Simgle molecule fluorescence spectoscopy of RNA hairpin loop folding

Stonehouse - Investigating the molecular interactions of the phi29 packaging motor

Stonehouse - Using in vitro selection to investigate RNA-protein interactions in picornaviruses

Thomas - High throughput techniques as a strategy to overcome crystal twinning

Thomas - Molecular mechanism of Staphylococcal plasmid transfer

Thomas - Investigating the interaction of quinolones with topoisomerase IV of Staphylococcus aureus

Thomas - Dimer specificity in a replication initiator protein

Thomson - Atomic force microscopy of DNA-protein interactions

Trinick - Class VI myosin

Trinick - Titin: studies of synthetic end-filaments

Trinick - EM image processing of filamentous structures - arthrin

Trinick - New apparatus for time-resolved electron cryo-microscopy

Warriner - Application of the Tmb acyl transfer auxilliary to the total chemical synthesis of Im7

Westhead - Design and implementation of a database to archive and aid the analysis of tissue microarray data

Whitehouse - Herpes viral-host cell interactions which regulate viral gene expression

Astbury Seminars 2003

Astbury Publications 2003