Astbury Centre for Structural Molecular Biology
Annual Report 2003

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The full 2003 report can be downloaded as an 11.5MB pdf filehere

Individual reports can be downloaded as pdf files using the links below

Director's statement for 2003 - Download

Ashcroft - Mass spectrometry facility

Ashcroft - Investigating the structure of monomeric and fibrillar beta2-microglobulin by limited proteolysis and mass spectrometry

Baron - Analytical centrifuge facility

Berry - Directed evolution of sialic acid synthesising enzymes

Berry - Directed evolution of dihydrodi picolinate synthase (DHDPS)

Berry - Directed evolution of an aldolase to enhance solubility

Findlay - Structure/function of G-protein coupled receptors (GPCRs)

Harris - Functional studies on the HIV-1 Nef protein

Harris - Structural and functional studies on the Hepatitis C virus non-structural NS5A protein

Homans - Thermodynamics of binding of 2-methoxy-3-isopropylpyrazine and 2-methoxy-3-isobutylpyrazine to the major urinary protein

Homans - Dissecting the cholera toxin-ganglioside GM1 interaction by isothermal titration calorimetry

Homans - The NMR solution structure of the VMA-7 subunit of the vacuolar H+-ATPase from Saccharomyces cerevisiae

Homans - Large scale millisecond inter-subunit dynamics in the B subunit homopentamer of the toxin derived form E. coli O157

Henderson - Collecting bacterial membrane proteins

Henderson - Characterisation of ligand binding to membrane transport proteins from Escherichia coli by solid state NMR

Hiscox - Characterisation of the coronavirus RNA binding protein

Jackson - Molecular modelling of SH2 domain-peptide interactions

Jackson - Flexligdock: A flexible ligand docking tool

Jackson - Searchable database containing comparisons of ligand binding sites at the molecular level for the discovery of similarities in protein function

Jackson - Structural modelling of protein-DNA interactions

Jaeger - Structural studies of RNA-directed RNA polymerases from Hepatitis C virus and related viruses

Jaeger - Biochemical and structural studies of Human Rhinovirus 3D polymerase

Jaeger - Functional and structural analysis of motif F in viral RNA polymerase

Jaeger - The mechanism of initiation in Hepatitis C virus RNA polymerase

Jaeger - Structural and biophysical studies on HIV-1 Nef

Kalverda - The NMR facility

McDowall - Quaternary structure and catalytic activity of the escherichia coli ribonuclease E amino-terminal catalytic domain

McDowall - Expanding the use of zymography by the chemical linkage of small, defined substrates to the gel matrix

McPherson - Enhanced enzyme properties by protein engineering of galactose oxidase

McPherson - Mobile TPQ cofactor and metals in E. coli copper amine oxidase

Nelson - Synthesis of screening substrates for the directed evolution of sialic acid aldolase

Parker - A combinatorial selective labelling methods for the assignment of backbone NMR resonances

Parker - Water as a conformational editor in protein folding

Phillips - Crystal structures of Qbeta RNA stemloop operators complexed with bacteriophage MS2 coat protein mutants; towards an explanation of binding discrimination

Phillips - Structural determination of human ketohexokinase

Phillips-Jones - Cytolysin gene expression in Enterococcus faecalis is regulated in response to aerobiosis conditions

Phillips-Jones - Expression, purification and characterisation of full-length, histidine protein kinase RegB (PrrB) from Rhodobacter sphaeroides

Phillips-Jones - Solution structure and DNA binding of the effector domain from the global regulator PrrA (RegA) from Rhodobacter sphaeroides: Insights into DNA binding specificity

Radford - Amyloid formation of peptides and point mutations of beta2-microglbulin

Radford - Investigating amyloid self-assembly by atomic force microscopy

Radford - Characterising the factors that describe the mechanical resistance of proteins

Radford - Mapping the folding landscapes of the four-helix proteins Im7 and Im9

Ranson - Cryo-EM of macromolecular complexes

Rowlands - Targeting the Hepatitis C virus ion channel p7 for anti-viral therapy

Rowlands - Discrimination among rhinovirus serotypes for a variant ICAM-1 receptor molecule

Smith - Atomic force microscopy of microdomains in lipid bilayers

Smith - The dynamic response of biomolecules

Smith - Fast folding of B domain of Protein A by laser induced temperature jump

Smith - Simgle molecule fluorescence spectoscopy of RNA hairpin loop folding

Stonehouse - Investigating the molecular interactions of the phi29 packaging motor

Stonehouse - Using in vitro selection to investigate RNA-protein interactions in picornaviruses

Thomas - High throughput techniques as a strategy to overcome crystal twinning

Thomas - Molecular mechanism of Staphylococcal plasmid transfer

Thomas - Investigating the interaction of quinolones with topoisomerase IV of Staphylococcus aureus

Thomas - Dimer specificity in a replication initiator protein

Thomson - Atomic force microscopy of DNA-protein interactions

Trinick - Class VI myosin

Trinick - Titin: studies of synthetic end-filaments

Trinick - EM image processing of filamentous structures - arthrin

Trinick - New apparatus for time-resolved electron cryo-microscopy

Warriner - Application of the Tmb acyl transfer auxilliary to the total chemical synthesis of Im7

Westhead - Design and implementation of a database to archive and aid the analysis of tissue microarray data

Whitehouse - Herpes viral-host cell interactions which regulate viral gene expression

Astbury Seminars 2003

Astbury Publications 2003