... understanding life in molecular detail

Dr Brian Jackson

Vector design, HT cloning, expression trials, protein purification


I run the Faculty’s Protein Production Facility (PPF), which provides a state-of-the-art service for purifying proteins. The Facility is brand new for 2016 and is currently in development. We are currently operational, but with limited capacity whilst development continues. We anticipate the Facility to be fully operational by Winter 2016.

Current major projects include:
  • Protein production from vector design to pure product
  • High-throughput cloning of genes of interest
  • Small scale protein expression trials
  • Expression from bacteria, insect and mammalian cells

~~For full details of the facility and the services and equipment available please see the main facility webpage on the Faculty of Biological Sciences website: http://www.fbs.leeds.ac.uk/facilities/protein/. Please note, this webpage is currently under development

Detailed research programme                  Close ▲
BJ.jpg

Research Facility Manager for Protein Production
PhD (University of East Anglia) 2004-2008


Postdoctoral Research Fellow (Leeds) 2008-2012
Wellcome Trust ISSF Junior Indep Research Fellow (Leeds) 2012-2016

Astbury 9.108f
School of Molecular and Cellular Biology

b.r.jackson@leeds.ac.uk
http://www.fbs.leeds.ac.uk/staff/profile.php?tag=Jackson_BR

Selected Publications

  1. Jackson BR*, Noerenberg M & Whitehouse A (2014). A novel mechanism inducing genome instability in Kaposi’s sarcoma-associated herpesvirus infected cells. PLOS Pathogens 10 e1004098. *corresponding author

  2. Jackson BR, Boyne JR, Noerenberg M, Taylor A, Hautbergue GM, Walsh R, Blackbourn DJ, Wilson SA & Whitehouse A (2011). An interaction between KSHV ORF57 and UIF provides mRNA-adapter redundancy in herpesvirus intronless mRNA export. PLOS Pathogens 7 e1002138.

  3. Tunnicliffe RB, Hautbergue GM, Kalra P, Jackson BR, Whitehouse A, Wilson SA & Golovanov AP (2011). Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins ICP27 and HVS ORF57. PLOS Pathogens 7 e1001244

  4. Boyne JR*, Jackson BR*, Taylor A, Macnab SA & Whitehouse A (2010). Kaposi’s sarcoma-associated herpesvirus ORF57 protein interacts with PYM to enhance translation of viral intronless mRNAs. EMBO Journal 29 1851-1864. *joint first authors