... understanding life in molecular detail

Dr Emma Hesketh

Cryo-EM, virology, single particle analysis, electron microscopy


I am responsible for the day-to-day operation of our Titan Krios electron microscopes optimised for cryo-electron microscopy (cryo-EM). My role includes providing guidance, training and advice to users (both internal and external) in areas covering sample preparation, optimisation, freezing, and image optimisation, using both single particle and tomographic methods. My main research focus is in structural virology. I use cryoEM and image processing methods to produce 3D structures of viruses.

Current major projects include:
  • Supporting users to utilise cryo-EM in their scientific research
  • Pump prime new cryoEM research
  • Investigate virus structure using cryoEM
  • Industrial liaison for cryoEM projects
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CryoEM Support Scientist

PDRA, (University of Leeds) 2015 - present

Roger Stevens 5.40
School of Molecular and Cellular Biology
0113 343 3384
E.L.Hesketh@leeds.ac.uk

Selected Publications

  1. Hesketh, E.L., Meshcheriakova, Y., Dent, K.C., Saxena, P., Thompson, R.F., Cockburn, J.J.B, Lomonossoff, G.P. & Ranson, N.A. (2015). Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Nature Comms., DOI:10.1038/ncomms10113

  2. Hesketh, E.L., Meshcheriakova, Y., Dent, K.C., Saxena, P., Thompson, R.F., Cockburn, J.J.B, Lomonossoff, G.P. & Ranson, N.A. (2015). Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Nature Comms., DOI:10.1038/ncomms10113

  3. Hesketh, E. L., Parker-Manuel, R. P., Chaban, Y., Satti, R., Coverley, D., Orlova, E. V., & Chong, J. P. J. (2015). DNA Induces Conformational Changes in a Recombinant Human Minichromosome Maintenance Complex. Journal of Biological Chemistry, 290(12), 7973–7979. DOI:10.1074/jbc.M114.622738