... understanding life in molecular detail

Dr Iain Manfield

Protein purification, HPLC, molecular interactions, DNA-protein interactions.


I run our Centre for Biomolecular Interactions technology facility, which provides access and training for equipment for purifying molecules and analysing the interactions between molecules. Biophysical analysis of molecules and interactions.

Current major projects include:
  • Purification using HPLC and FPLC
  • Analysis of molecular interactions and protein properties
  • Analysis of DNA-protein interactions

Iain runs the Centre for Biomolecular Interactions (link), with Amy Magoolagan. Iain's background is in the analysis of DNA-protein interactions and protein over-expression and purification.

Detailed research programme                  Close ▲
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Technology facility manager



Post doc, Biophysics Department, Portsmouth University, 1992 - 1998
Post doc. Centre for Plant Sciences, University of Leeds, 1998 - 2008

Astbury 9.108
School of Molecular and Cellular Biology
0113 343 7279
http://www.fbs.leeds.ac.uk/staff/profile.php?un=bm

Selected Publications

  1. D. Roeland Boer1,2,†, Alejandra Freire-Rios3,†, Willy van den Berg3,†, Terrens Saaki3, Iain W. Manfield4, Stefan Kepinski4, Irene López-Vidrieo5, Jose Manuel Franco5, Sacco C. de Vries3, Roberto Solano5, Dolf Weijers3,* and Miquel Coll1,2,* Structural basis for DNA binding specificity by the auxin-dependent ARF transcription factors. Cell, accepted for publication February 2014.

  2. Lee KJ; Cornuault V; Manfield IW; Ralet MC; Knox JP Multiscale spatial heterogeneity of pectic rhamnogalacturonan-I (RG-I) structural features in tobacco seed endosperm cell walls. Plant J -, 2013
    DOI:10.1111/tpj.12263

  3. Gruszka DT; Wojdyla JA; Bingham RJ; Turkenburg JP; Manfield IW; Steward A; Leech AP; Geoghegan JA; Foster TJ; Clarke J; Potts JR Staphylococcal biofilm-forming protein has a contiguous rod-like structure. Proc Natl Acad Sci U S A 109 E1011-E1018, 2012
    DOI:10.1073/pnas.1119456109

  4. Manfield IW; Devlin PF; Jen CH; Westhead DR; Gilmartin PM Conservation, convergence, and divergence of light-responsive, circadian-regulated, and tissue-specific expression patterns during evolution of the Arabidopsis GATA gene family Plant Physiology 143 941-958, 2007
    DOI:10.1104/pp.106.090761