... understanding life in molecular detail

Prof John Ladbury

Structure/function, Protein signalling, Drug discovery, Cellular mechanisms


Focusing on early signalling events we are exploring the structural, biophysical, and cellular outcomes of protein complex formation at receptors. Perturbation of these complexes by inhibiting assembly or modifying the time course reveals how the exquisite sensitivity of early signalling complex formation can ensure specificity and lead to disease.

Current major projects include:
  • aberrancies in signaling lead to cancer
  • signaling in growth factor-deprived cells
  • biophysical characterization of complex formation
  • Thermodynamics in drug development

Professor Ladbury’s independent research is strongly influenced by his background in biophysical chemistry. He has published seminal investigations on the application of biophysical data to the understanding of signal transduction and in drug development. This experience was also exploited for investigating the mechanisms by which proteins are able to propagate and maintain mutually exclusive signals in mammalian cells. This work questioned existing dogma regarding specificity in interactions involved in tyrosine kinase-mediated signal transduction. His observations in this area led him to investigate the role of multi-protein complexes in signalling pathways. This has culminated in the development of a probabilistic view of signalling regulated by control and switching mechanisms, perturbation of which can lead to disease states such as cancer. The Ladbury laboratory adopts methods including cell biology, biophysical instrumental techniques and high resolution structure determination. He has combined the use of cell-based fluorescent life time imaging microscopy with in vitro measurement of biomolecular interactions to investigate the formation protein ensembles on plasma membrane receptors. Professor Ladbury has also maintained an interest in the area of structure-based drug design and has pioneered approaches to integrate thermodynamic parameterisation into this process. Professor Ladbury has always been an enthusiastic collaborator and has published with many of the leading scientists in his field. He has also worked closely with industrial partners and has received research funding from UCB/Celltech and AstraZeneca. In his previous role in the USA he successfully developed the interface between biophysics/structural biology and clinical scientists at MDACC. This activity in which researchers are encouraged to include biophysical/structural data in their interpretation of clinical outcomes will continue at the University of Leeds.

 

Detailed research programme                  Close ▲
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Dean of the Faculty of Biological Sciences
BSc. (London) MSc, PhD (MoD/Greenwich)
Postdoc- Yale University 1990-93 and Harvard University Medical School 1993; Senior Research Scientist- New York University Medical Center 1994; Wellcome Trust Research Career Development Fellow (1994-1998); Wellcome Trust Senior Research Fellow (1998-2008); University of Texas STARS Award (2008); IUPAC Fellow (2008-2012)

Wellcome Trust Research Fellow, University of Oxford, 1995-1996
Professor of Molecular Biophysics, UCL, 1996-2008
Endowed Professor of Cancer Research, MD Anderson Cancer Center 08-14
Director Center for Biomol. Struct. & Funct. MD Anderson Cancer Cent,

LC Miall 7.07
School of Molecular and Cellular Biology
011334335669
j.e.ladbury@leeds.ac.uk

Selected Publications

  1. Ahmed, Z., Timsah, Z., Suen, K. M., Cook, N. P., Lee IV, G. R., Lin, C. –C., Gagea, M., Marti, A. A. & Ladbury, J. E. (2015)
    Grb2 monomer-dimer equilibrium determines normal versus oncogenic function. Nature Communications 6, 7354.

  2. Timsah, Z., Ahmed, Z., Lin, C. –C., Melo, F. A., Stagg, L. J., Leonard, P. G., Jeyabal, P., Berrout, J., O.Neil, R. G., Bogdanov, M. & Ladbury, J. E. (2014) Competition between Grb2 and Plcγ1 for binding to FGFR2 regulates constitutive phospholipase activity and invasive response. Nature Structural and Molecular Biology 21, 180-188.

  3. Chan, C. –H., Morrow, J. K., Li, G. –F., Gao, Y., Jin, G., Moten, A., Stagg, L. J., Ladbury, J. E., Cai, Z., Xu, D., Logothetis, C. J., Hung, M. –C., Zhang, S. & Lin, H. –K. (2013)  Pharmacological inactivation of Skp2 SCF ubiquitin ligase restricts cancer stem cell traits and cancer progression.  Cell 154, 556-568.

  4. Lin, C-. C., Melo, F. A., Ghosh, R. Suen, K. M., Stagg, L. J., Kirkpatrick, J., Arold, S. T., Ahmed, Z. & Ladbury, J. E. (2012) Inhibition of basal FGF receptor signaling by dimeric Grb2. Cell 149, 1514-1524.