... understanding life in molecular detail

Dr James Ault

Mass spectrometry, ion mobility spectrometry, protein analysis, protein conformation


James is the manager of the Mass Spectrometry Facility providing a biomolecular analysis service for a wide variety of research groups within Leeds, at other institutions and industry. Analyses available include intact accurate mass determination, protein identification and native-like mass spectrometry for determining complex binding and stoichiometry. Please see www.fbs.leeds.ac.uk/facilities/ms for more information.

Current major projects include:
  • Probing protein-ligand interactions using IMS-MS
  • Structural proteomics methods for determing PTMs
  • Methods development for MS service analyses

Please see www.fbs.leeds.ac.uk/facilities/ms for more information about James’ work in the MS Facility.

Detailed research programme                  Close ▲
JRA.jpg

Mass Spectrometry Facility Manager
MChem (Mancheseter), PhD (York)


Post-doc Research Fellow (Buck Institute for Research on Aging) 2007

Astbury 9.107
School of Molecular and Cellular Biology
0113 343 8188
j.r.ault@leeds.ac.uk

Selected Publications

  1. Eves-van den Akker S; Lilley CJ; Ault JR; Ashcroft AE; Jones JT; Urwin PE The feeding tube of cyst nematodes: Characterisation of protein exclusion PLOS One accepted 2014

  2. Timms N; Windle CL; Polyakova A; Ault JR; Trinh CH; Pearson AR; Nelson A; Berry A Structural insights into the recovery of aldolase activity in N-acetylneuraminic acid lyase by replacement of the catalytically active lysine withγ-thialysine by using a chemical mutagenesis strategy. Chembiochem 14 474-481, 2013
    DOI:10.1002/cbic.201200714

  3. Wilson AJ; Ault JR; Filby MH; Philips HI; Ashcroft AE; Fletcher NC Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics. Org Biomol Chem 11 2206-2212, 2013
    DOI:10.1039/c3ob26251k

  4. Phillips HIA; Chernikov AV; Fletcher NC; Ashcroft AE; Ault JR; Wilson AJ; Filby MH The use of electrospray mass spectrometry to determine speciation in a dynamic combinatorial library for anion recognition Chemistry - A European Journal 18 13733-13742, 2012
    DOI:10.1002/chem.201201302