... understanding life in molecular detail

Dr Peiyi Wang

Cryo-EM, Membrane protains, Single particle analysis, 3D structure reconstrcution


Cryo-EM is becoming popularity in structural biology. This is because the technique allows observing specimens in their native environment without being stained or fixed, which can lead to functionally irrelevant conformational changes. It suits for studying both the structure and dynamics of large proteins and protein complexes. The resolution of cryo-EM images is improving significantly due to the hardware development in recent year. A virus structure obtained by cryo-EM is already at a resolution 3.3 Å that can be interpreted in terms of an atomic model.

 

Current major projects include:
  • Optimisation of amorphous carbon films
  • 3D data collection and reconstruction of virus by Tomography
  • Particle structure analysis on the function of the Hcp GP96 protein
  • Electron crystallography of 2D crystal analysis on membrane proteins

My research is focusing on using state-of-the-art electron cryomicroscopy to understand the structure and functions of protein and macromolecular complexes. I am also interesting to develop and apply the new methodology in the field.

 

Detailed research programme                  Close ▲
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Cryo-EM Specialist, Leeds, 2007 - present
BSc. MSc. PhD.

Postdoc (Southamptom) 1996 - 1997
Postdoc (Sheffield) 1998 - 1999
Manager of Cryo-EM facility (Sheffield) 2000 - 2006

Manton Building 5.93e
School of Molecular and Cellular Biology
0113 343 3384
fbspw@leeds.ac.uk

Selected Publications

  1. N. Hondow, R. Brydson, P. Wang, M. D. Holton, M. Rowan Brown, P. Rees, H. D. Summers and A. Brown, Quantitative characterization of nanlparticle agglomeration within biological media, J Nanopart Res (2012), 14:977

  2. P. Qian, H. A. Addlesee, A. V. Ruban, P. Wang, P. A. Bullough and C. Neil Hunter. A reaction center-light-harvesting 1 complex (RC-LH1) from Rhodspirillum rubrum mutant with altered esterifying pigments. J. Biol. Chem., 2003, vol. 278, no. 26, 23678-23685

  3. S.J. Jamieson, P. Wang, P. Qian, J.Y. Kirkland, M.J. Conroy, C. N. Hunter and P. A. Bullough. Projection structure of the photosynthetic reaction centre-antenna complex of Rhodospirillum rubrum at 8.5 Å resolution. EMBO J. (2002) 15 3927-3935